Lecture 3: Laboratory Tests of Hemostasis, Part 2
Gerald A Soff MD
Presentation Date:
September 18, 2025
> Laboratory testing of coagulation provides the tools to diagnose patients with thrombosis and hemorrhage.
> In this presentation, we will review the state-of-the-art for testing for acquired and hereditary hypercoagulable states.
Gerald A Soff MD
Chief, Classical Hematology Service
University of Miami Health System/Sylvester Comprehensive Cancer Center
Email: gas199@miami.edu
Disclosures for Gerald A Soff MD.
Research Support (Past 2 years):
Amgen, Sobi/Dova Pharmaceuticals
Anthos Therapeutics
Data Safety Monitoring Committee
Alpine Immune Sciences
Advisory Boards (Past 2 years)
Sanofi , Novartis
Learning Objectives: Part 2: Laboratory Tests of Thrombotic Disease.
> Describe the pathophysiology of heparin-induced thrombocytopenia and discuss the appropriate use of screening and confirmatory tests.
> Discuss the test principle and interpretation of confirmatory tests of the Lupus Anticoagulant assays
> List appropriate laboratory evaluation of patients with suspected inherited thrombophilia.
> Discuss the effect of therapeutic anticoagulants on the reliability of results of clot based, chromogenic, immunologic and molecular tests.
> Factor V: Leiden polymorphism (G1691A (R506Q) is associated with activated protein C resistance, prolonged/enhanced activity of Factor Va, and thrombotic tendency.
> It is the most common hereditary hypercoagulable state.
> Factor V: Leiden is evaluated by PCR assay.